Effect of Flushing the Endometrial Cavity With Follicular Fluid on Implantation Rates in Sub-Fertile Women Undergoing Invitro Fertilization: A Randomized Clinical trial
-
Kobra Hamdi
,
Mohammad Nouri
,
Sara Farzaneh
,
Mohammad Mirza-Aghazadeh-Attari
,
Mohammad Naghavi-Behzad
,
Sahar Mohammadi
Abstract
Objective: To examine the effectiveness of intrauterine injection of follicular fluid in in vitro fertilization (IVF).
Materials and methods: A parallel randomized control clinical study was conducted on 110 patients attending Al–Zahra Educational-Medical Center of Tabriz University of Medical Sciences. Female candidates for IVF were categorized into intervention (n = 55) and control (n = 55) groups using Randlast software (version 1.2). Following an identical protocol of gonadotropin-releasing hormone (Gnrh) antagonist stimulating ovulation, in the intervention group a total of 2mL of follicular fluid was injected as intrauterine after the accomplishment of follicular puncture. Embryo transfer was carried out after 2-3 days. The rates of implantation, as well as chemical and clinical pregnancy were compared between the two groups.
Results: There were no significant differences in chemically proven pregnancy (19 in intervention group (34.5%) vs. 23 (41.8%) in control group p = 0.43), or in clinical pregnancy (30.9% vs. 38.2%, respectively p = 0.42), and in implantation rates (11.52 ± 2.57 % (range, 0-66.7) vs 18.79 ± 3.72 % (range,
0-100), respectively).
Conclusion: Injection of follicular fluid into the uterine cavity in candidates for IVF neither improves nor adversely affects the outcome of the therapy.
Fritz MA, Speroff L. Clinical gynecologic endocrinology and infertility. 8th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins; 2011. x, 1439 p. p.
Mostafa Gharabaghi P, Abdollahifard S, Feizina S. Predicting Factors of Ovarian Response during Invitro Fertilization (IVF) Cycle in Infertility Center of Alzahra Medical Journal of Tabriz University of Medical Sciences and Health Services. 2011;33(4):7-11.
Taghavi S, Ghojazadeh M, Azami-Aghdash S, Alikhah H, Bakhtiarzadeh K, Azami A, et al. Assessment of mothers’ satisfaction with the care of maternal care in Specialized Educational-Medical Centers in obstetrics and gynecological disease in Northwest, Iran. Journal of Analytical Research in Clinical Medicine. 2015;3(2).
Moragianni VA, Penzias AS. Cumulative live-birth rates after assisted reproductive technology. Current opinion in obstetrics & gynecology. 2010;22(3):189-92.
Stern JE, Brown MB, Luke B, Wantman E, Lederman A, Missmer SA, et al. Calculating cumulative live-birth rates from linked cycles of assisted reproductive technology (ART): data from the Massachusetts SART CORS. Fertility and sterility. 2010;94(4):1334-40.
Boivin J, Griffiths E, Venetis CA. Emotional distress in infertile women and failure of assisted reproductive technologies: meta-analysis of prospective psychosocial studies. Bmj. 2011;342:d223.
Hamdi K, Vaezi M, Dagigazar B, Sadagiani MM, Farzadi L, Pashaei-Asl M. Association between Thrombophilia and Repeated Assisted Reproductive Technology Failures. Advanced pharmaceutical bulletin. 2012;2(2):233.
Hashish NM, Badway HS, Abdelmoty HI, Mowafy A, Youssef MA. Does flushing the endometrial cavity with follicular fluid after oocyte retrieval affect pregnancy rates in subfertile women undergoing intracytoplasmic sperm injection? A randomized controlled trial. European journal of obstetrics, gynecology, and reproductive biology. 2014;176:153-7.
Farzadi L, Nouri M, Ghojazadeh M, Mohiti M, Aghadavod E. Evaluation of ovarian reserve after laparoscopic surgery in patients with polycystic ovary syndrome. BioImpacts: BI. 2012;2(3):167.
Moslemi F. Apgar scores and cord blood gas values on neonates from cesarean with general anesthesia and spinal anesthesia. Journal of Analytical Research in Clinical Medicine. 2013;2(1).
Zarei A, Parsanezhad ME, Younesi M, Alborzi S, Zolghadri J, Samsami A, et al. Intrauterine administration of recombinant human chorionic gonadotropin before embryo transfer on outcome of in vitro fertilization/ intracytoplasmic sperm injection: A randomized clinical trial. Iranian journal of reproductive medicine. 2014;12(1):1-6.
Potdar N, Gelbaya T, Nardo LG. Endometrial injury to overcome recurrent embryo implantation failure: a systematic review and meta-analysis. Reproductive BioMedicine Online. 2012;25(6):561-71.
Maccio A, Mantovani G, Turnu E, Artini P, Contu G, Volpe A. Preovulatory human follicular fluid in vitro inhibits interleukin (IL)-1 alpha, IL-2, and production and expression of p55 chain IL-2 receptor of lymphomonocytes. Fertility and sterility. 1994;62(2):327-32.
Levi Setti PE, Colombo GV, Savasi V, Bulletti C, Albani E, Ferrazzi E. Implantation failure in assisted reproduction technology and a critical approach to treatment. Annals of the New York Academy of Sciences. 2004;1034:184-99.
Tehraninejad ES, Tanha FD, Ghajarzadeh M, Zandieh Z, Aziminekoo E, Zanjani HR. Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial. Archives of gynecology and obstetrics. 2012;285(4):1167-71.
Prapas Y, Petousis S, Panagiotidis Y, Gullo G, Kasapi L, Papadeothodorou A, et al. Injection of embryo culture supernatant to the endometrial cavity does not affect outcomes in IVF/ICSI or oocyte donation cycles: a randomized clinical trial. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2012;162(2):169-73.
Zhu W, Li X, Fu Z, Tang Z, Chen X, Zhou Y, et al. Injection of day 2 embryo culture supernatant into the uterine cavity did not improve the pregnancy rate of day 3 embryo transfer in patients who underwent in vitro fertilization-embryo transfer: a randomized clinical trial. Fertility and sterility. 2010;93(7):2216-21.
Berkkanoglu M, Isikoglu M, Seleker M, Ozgur K. Flushing the endometrium prior to the embryo transfer does not affect the pregnancy rate. Reprod Biomed Online. 2006;13(2):268-71.
Goto S, Kadowaki T, Hashimoto H, Kokeguchi S, Shiotani M. Stimulation of endometrium embryo transfer (SEET): injection of embryo culture supernatant into the uterine cavity before blastocyst transfer can improve implantation and pregnancy rates. Fertility and sterility. 2007;88(5):1339-43.
Goto S, Kadowaki T, Hashimoto H, Kokeguchi S, Shiotani M. Stimulation of endometrium embryo transfer can improve implantation and pregnancy rates for patients undergoing assisted reproductive technology for the first time with a high-grade blastocyst. Fertility and sterility. 2009;92(4):1264-8.
Somigliana E, Vigano P, La Sala GB, Balasini M, Candiani M, Incerti L, et al. Follicular fluid as a favourable environment for endometrial and endometriotic cell growth in vitro. Human reproduction. 2001;16(6):1076-80.
Guzeloglu-Kayisli O, Kayisli UA, Taylor HS. The role of growth factors and cytokines during implantation: endocrine and paracrine interactions. Seminars in reproductive medicine. 2009;27(1):62-79. Epub 2009/02/07.
Mehta BN, Chimote NM, Chimote MN, Chimote NN, Nath NM. Follicular fluid insulin like growth factor-1 (FF IGF-1) is a biochemical marker of embryo quality and implantation rates in in vitro fertilization cycles. Journal of human reproductive sciences. 2013;6(2):140-6. Epub 2013/10/02.
Feng R, Sang Q, Zhu Y, Fu W, Liu M, Xu Y, et al. MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro. Scientific reports. 2015;5:8689.
Vujisic S, Lepej SZ, Emedi I, Bauman R, Remenar A, Tiljak MK. Ovarian follicular concentration of IL-12, IL-15, IL-18 and p40 subunit of IL-12 and IL-23. Human reproduction. 2006;21(10):2650-5.
| Files | ||
| Issue | Vol 12, No 4 (December 2018) | |
| Section | Original Articles | |
| Keywords | ||
| Endometrial Cavity Follicular Fluid Invitro Fertilization Implantation | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
