Fertility Enhancing Activities of Bioactive Components of Cochlospermum planchonii Rhizome on Cisplatin Induced Reproductive Dysfunctions in Sprague–Dawley Rats
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Abstract
Objective: Cisplatin has been established to cause reproductive dysfunction; Cochlospermum planchonii is globally used in folklore medicine and has numerous therapeutic benefits. This study focused on fertility enhancing activities of Cochlospermum planchonii (Cp) on cisplatin-induced reproductive dysfunctions.
Materials and methods: Total of 30 male and 30 female adult Sprague-Dawley rats were used for this study. The male rats randomly assigned into Group A (control) was given normal saline 2 ml/kg, Group B, C, D and E rats received(single dose of 8 mg/kg Cisplatin (i.p.) on the first day), (500 mg/kg body weight (bwt) of Cponce once daily for 14 days), (single dose of 8 mg/kg Cisplatin (i.p.) on 1st day followed by 500 mg/kg bwt of Cp once daily for 14 days) and (single dose of 8 mg/kg Cisplatin on first day followed by 50 mg/kg vitamin C for 14 days). Parameters tested include reproductive hormones, testicular histology, testicular antioxidants, semen parameters and fertility test.
Results: Histological profile of the testes revealed derangement of the testis cytoarchitecture; Seminiferous epithelium, body, testes, accessory sex organs weight and sperm parameters, were significantly reduced (p <0.05). Hormonal assay showed significant changes in testosterone (p< 0.05) while luteinizing hormone and follicle stimulating hormone remained unchanged following cisplatin administration and a marked improvement was observed after Cochlospermum planchonii administration. Similarly, Cochlospermum planchonii improved the reduction of antioxidant parameters (SOD, CAT, GPx and GSH) and the increased MDA caused by cisplatin ingestion.
Conclusion: Cochlospermum planchonii may thus offer protection against free radical mediated oxidative stress of rats with cisplatin induced reproductive dysfunction.
World Health Organization, Progress in Reproductive Health Research, World Health Organization, 2003, No 63.
Chia SE, Lim ST, Tay SK, Lim ST. Factors associated with male infertility: acase control study of 218 infertile and 240 fertile men, Br. J. Obstet. Gynaecol. 2000; 107:55–61.
Shalaby MA, Mouneir SM. Effect of zingiber officinale roots and cinnamon zeylanicum bark on fertility of male diabetic rats. Glob Veter. 2010;5(6):341–7.
Donmez DB, Bozdogan S. Effect of Sodium Selenite on Testicular Damage Induced by Cisplatin in Adult Male Rats. Biol Med. 2014;6:209.
Amin A, Buratovich MA. New platinum and ruthenium complexes-the latest class of potential chemotherapeutic drugs-a review of recent developments in the field. Mini Rev. Med. Chem. 2009;9(13): 1489–1503.
Bae WK, Hwang JE, Shini HI, Cho SH, Lee KH, Han HS, Song EK, Yun HJ, Cho IS, Lee JK, Lim SC, Ching WK, Chamg IJ. Multicenter Phase II study of weekly docetaxel. Cisplatin, and S-1 (IPS) induction chemotherapy for locally advanced squamous cell cancer of the head and neck. BMC.Cance. 2013;13:102.
Hotta K, Takigawa N, Hisainoto-Sato A, Ichthar LE, Kudo K, Uchida K, Yanase-Nakamura K, Tanaka H, Kato Y, Tabata M,Taninoto M, Kiua K . Reappraisal of short-term low-volume hydration in cisplatin-based chemotherapy: results of a prospective feasibility study in advanced lung cancer in the Okayama Lung Cancer Study Group Trial 1002. Jpn. J. Clin. Oncol.2013; 43:1115–1121
Ozkol H, Musa D, Tuluce Y, Koyuncu I. Ameliorative influence of Urticadioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug Chem. Toxicol. 2012; 35:251–257.
Furukawa S, Hayashi, S, Usuda K, Abe M, Hagio S, Ogawa I. Effect of cisplatin on rat placenta development. Exp. Toxicol. Pathol. 2013;65:211–217.
El-Sawaihi MM, Ahmed LA. Exploring the protective role of apocynin, a specific NADPH oxidase inhibitor, in cisplatin-induced cardiotoxicity in rats. Chem. Biol. Interact., 2014;207:58–66.
Beytur AO, Ciftci FO, Oguzturk H, Yilmaz F. Montelukast attenuates side effects of cisplatin including testicular, spermatological, and hormonal damage in male rats. Cancer Chemo ther. Pharmacol.2012;69(1): 207–213.
Fung C, Vaughn DJ. Complications associated with chemotherapy in testicular cancer management. Nat. Rev. Urol. 2011;8(4): 213–222.
Rezvanfar MA, Rezvanfar MA, Shahverdi AR. Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicol. Appl. Pharmacol. 2013; 266(3): 356–365.
Maines MD, Sluss PM, Iscan M. Cis-Platinum-Mediated Decrease in Serum Testosterone is Associated with Depression of Luteinizing Hormone Receptors and Cytochrome P-450scc in Rat Testis. Endocrinology.1990;126(5): 2398–2406.
Narayana KM, Al-Bader, Mousa A, Khan KM. Molecular effects of chemotherapeutic drugs and their modulation by antioxidants in the testis. Eur. J. pharmacol.2012; 674(2): 207–216.
Ahmed EA, Omar HM, Elghaffar S, Ragb SM, Nasser AY. The antioxidant activity of vitamin C, DPPD and L-cysteine against Cisplatin-induced testicular oxidative damage in rats.Food Chem. Toxicol.2011;49(5): 1115–1121.
Boekelheide K. Mechanisms of toxic damage to spermatogenesis. J. Natl. Cancer Inst. Monogr., 2005;34: 6–8.
Ilbey YO, Ozbek E, Cekmen M, Simsek A, Otunctemur A, Somay A. Protective effect of curcumin in cisplatin-induced oxidative injury in rat testis: mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways. Hum. Reprod. 2009a; 24(7): 1717–1725.
Dragsted LO. Antioxidant actions of polyphenols in humans. Int J VitamNutr Res. 2003;73(2):112–9.
Scalbert A, Manach C, Morand C, Remesy C, Jimenez L. Dietary polyphenols and the prevention of diseases. Crit Rev Food SciNutr.2005;45(4):287–306.
Burkill HM. The useful plants of West Africa, Vol. (1). Royal Botanical Gardens, 1985;pp. 386–387.
Igoli SO, Ogeji OG, Tor-Anyiin TA, Igoli NP. Traditional medical practice amongst the Igede people of Nigeria. Afr. J. Trad. Compl.Altern. Med.2005 2(2): 134–152.
Benoit-Vical F, Valentin A, Da B, Dakuyo Z, Descamps L, Mallié MN (Cochlospermum planchonii) versus chloroquine for treatment of uncomplicated Plasmodium falciparum malaria. J. Ethnopharmacol. 2003;89(1): 111–114.
Anthony JP, Fyfe L, Smith H. Plant active components, a resource for antiparasitic agents. Trends Parasitol.2005;21: 462–468.
Nduagu C, Ekefan EJ, Nwankiti AO. Effect of crude plant extracts on growth of Colletotrichumcapsici(Synd) Butler & Bisby, causal agent of pepper anthracnose. J. Appl. Biosci. 2008;6(2): 184–190.
Mann A, Gbate M, Umar AN. Medicinal and Economic plants of Nupeland. Jube-Evans Books and Publications, Bida, Nigeria.2003;p. 64.
Atawodi SE. Comparative in vitro trypanocidal activities of petroleum ether, chloroform, methanol and aqueous extracts of some Nigerian savannah plants. Afr. J. Biotechnol. 2005;4(2): 177–182.
Anaga AO, Oparah NO. Investigation of the methanol root extract of Cochlospermum planchonii for pharmacological activities in vitro and in vivo. Pharm. Biol. 2009;47(11): 1027–1034.
Iweala EJ, Okeke CU. Comparative study of the hypoglycemic and biochemical effects of Catharanthusroseus(Linn) g. apocynaceae (Madagascar periwinkle) and chlorpropamide (diabenese) on alloxan-induced diabetic rats. BIOKEMISTRI.2015; 17:149–156.
Khan MRI, Islam MA, Hossain MS, Asadujjaman M, Wahed MII, Rahman BM et al. Antidiabetic Effects of the Different Fractions of Aqueous Extracts of Ocimum sanctum in Normal and Alloxan Induced Diabetic Rats. J. Sci. Res. 2010; 2:158–168.
Soni A, Sosa S. Phytochemical analysis and free radical scavenging potentialof herbal and medicinal plant extracts, J. Pharmacogn. Phytochem. 2013;2:22–29.
Yakubu MT, Akanji MA, Oladiji AT, Olatinwo AO, Adesokan AA, Yakubu MO, et al. Effect of Cnidoscolousaconitifolius (Miller) I.M. Johnston leaf extract on reproductive hormones of female rats. Iran J Reprod Med. 2008;6:149–155.
World Health Organization. Laboratory Manual for the Examina¬tion of Human Semen and Semen-Cervical Mucus Interaction.4 ed¬ NewYork: Cambridge University Press; 1999.
Deniz S, Erdineler AS, Figen I, Tanju B, Gulden G. Lipid peroxidation and oxidant status in experimental animals: effect of aging and hypercholestrolemic diet. Clin.Acta.1997; 265: 77–84.
Beutler EO, Duron, Kelly BM. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 1963;61: 882–888.
Kayode O, Olusegun J, Adesanya A, Idowu O, Josiah S, Tolupe O, Effects of crude ethanolic extract of Garcinia cambogia on the reproductive system of male wistar rats (Rattusnovergicus), Afr. J. Biotechnol. 2007;6:1236–1238.
Howard CV, Reed MG. Unbiased Stereology: Three-Dimensional Measurement in Microscopy, Second edition, Abingdon: GarlandScience/BIOS Scientific, 2005
Baines H, Nwagwu MO, Hastie GR, Wiles RA, Ebling F. Effects of estradioland FSH on maturation of the testis in the hypogonadal (hpg) mouse, Reprod.Biol. Endocrinol. 2008;6: 4
Ligha AE, Bokolo B, Didia BC. Antifertility potential of Mentronidazole in Male Wistar Rats. Pakistan Journal of Biological Sciences.2012; 15:224–230.
National Institutes of Health Guide for the Care and Use of Laboratory Animals: DHEW Publication (NIH), revised. Office of Science and Health Reports, DRR/NIH, Bethesda, USA, 1985.
Vaidya AB, Sirsat SM, Doshi JC, Antarkar, DS. Selected medicinal plants and formulation as hepatobiliary drugs: An overview.Indian J ClinPharmacol.Ther. 1996;17:7.
Dilek BD, Seren B. Effect of Sodium Selenite on Testicular Damage Induced by Cisplatin in AdultMale Rats. Biol Med 2014;6(3):1–4
Zhang X, Yamamoto N, Soramoto S, Takenaka I. Cisplatin induced germ cell apoptosis in mouse testes. Arch Androl2001;46: 43–49.
Kim Boekelheide. Mechanisms of Toxic Damage to Spermatogenesis. Journal of the National Cancer Institute Monographs2005:34
Abu AH, Ochalefu DO, Ibrahim A. Aqueous ethanolic extract of Cochlospermum planchonii rhizome enhances spermatogenesis in male albino rats. African Journal of Biotechnology. 2012; 11(53): 11636–11639
Choudhary A, Steinberger E. Effect of a-reduced androgen on sex accessory organs initiation and maintenance of spermatogenesis in the rat. Biol. Reprod.1975; 12: 609-617.
Shittu LAJ, Bankole MA, Oguntola JA,Ajala O, Shittu RK, Ogundipe OA, Bankole MN, Ahmed T, Ashiru OA. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult maleSprague Dawley rat. Scientific Research and Essay. 2007;2 (8): 319–324.
Alvarez JG, Storey BT, Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod.Devel.1995;42: 334–346.
Saleh RA, Agarwal A, Oxidative stress and male infertility: from researchbench to clinical practice, J. Androl. 2002; 23:737–752.
Armstrong JS, Rajasekaran M, Chamulitrat W,Gatti P, Hellstrom WJ, Sikka SC. Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism, Free Rad. Biol. Med.1999;26: 869–880.
de Lamirande E, Gagnon C, Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum. Reprod. 1995;10:15–21.
Rodrigues HG, Diniz YS, Faine LA, Galhardi CM, Burneiko RC, Almeida JA, Ribas BO, Novelli EL. Antoxidant effect of Saponin: potential action of a soybean flavonoid on glucose tolerance and risk factors for atherosclerosis, Int. J. food Sci. Nutr. 2005;56 :79–85.
Agarwal A, Ikemoto I, Loughlin KR. Relationship of sperm parameters with levels of reactive oxygen species in semen specimens. J Urol 1994;152:107–110.
Gomez E, Irvine DS, Aitken RJ. Evaluation of a spectrophotometric assay from the measurement of malondialdehyde and 4hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function. Int J Androl 1998;21:81–94.
Trush MA, Mimnaugh EG, Gram TE. Activation of pharmacologic agents to radical intermediates. Implications for the role of free radicals in drug action and toxicity.Biochem.Pharmacol.1982;31, 3335–3346.
Zhifei Liu, Yingbiao Sun, Li Su, Yifan Sun, Shibo Kong, xuhong Chang, Fang Guo,
Wei Li, Junjie G, Jin Li . Effects of cisplatin on testicular enzymes and Sertoli cell
function in rats.Fundamental Toxicological Sciences .2015;2(4): 137–145.
Ahmed HE, Noha FA, Ola MA, Hala MF, Ezz-El-Din SE. Captopril and telmisartan ameliorate cisplatin-induced testicular damage in rats via anti-inflammatory and antioxidant pathways. International Journal of Scientific and Research Publications. 2016;6(1): 408–420
KilarkajeN, Mousa AM, AL-Bader MM, Khan KM . Antioxidants enhance the recovery of three cycles or bleomycin, etoposide, and cisplatin-induced testicular dysfunction, pitutary-testicular axis, and fertility in rats. Fertil. Steril.2013;100:1151–1159.
Adejuwon SA, Femi-Akinlosotu OM, Omirinde. Cisplatin-induced testicular dysfunction and its amelioration by Launaeataraxacifolia leaf extract. Andrologia. 2015; 47(5): 553–559.
Salem EA, Salem NA, Maarouf AM, Serefoglu EC, Hellstrom WJ. Selenium and lycopene attenuate cisplatin-induced testicular toxicity associated with oxidative stress in Wistar rats. Urology. 2012;79(5): 1181–1186.
Guyton AC, Hall JE. Textbook of medical physiology, 11th edition, Elsevier saunders; 2006
Joensen UN, Jorgensen N, Meyts ER, et al. Testicular Dysgenesis syndrome and leydig cell function. Basic and Clinical Pharmacology and Toxicology 2008;102:155–161.
Zirkin BR, Spermatogenesis its regulation by estosterone and FSH, Semin. Cell Dev. Biol. 1998;9: 417–421.
Aitken Roman RJSD. Antioxidant systems and oxidative stress in the testes, Oxid.Med. Cell Longevity.2008; 1:15–24.
Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O et al. Vitamin C as an antioxidant: Evaluation of its role in disease prevention. J. Am. Coll. Nutr. 2003;22: 18–35.
Colagar AH, Marzony ET. Ascorbic acid in human seminal plasma: Determination and its relationship to sperm quality. J. Clin. Biochem.Nutr.2009;45: 144–149.
Agarwal A, Sharma RK, Nallella KP, Thomas Jr AJ., Alvarez JG , Sikka SC. Reactive oxygen species as an independent marker of male factor infertility. Fertil.Steril.2006;86: 878–885.
Turner TT, Lysiak JJ. Oxidative stress: A common factor in testicular dysfunction. J. Androl. 2008;29: 488–498.
Aprioku JS, Ibeachu C, Ijeomah MA . Protective Effect of Ascorbic Acid on Cimetidine-Induced Reproductive Toxicity in Male Wistar Rats. Journal of Pharmacology and Toxicology.2014; 9: 46–54.
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| Issue | Vol 12, No 3 (September 2018) | |
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| Cisplatin Cochlospermum Planchonii Oxidative Stress Hormone Rats Fertility | ||
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