Role of Aqueous Crude Leaf Extract of Senecio Biafrae Combined With Zinc on Testicular Function of Adult Male Sprague Dawley Rats
,
Abstract
Objective: To determine the role of aqueous crude leaf extract of Seneciobiafrae (SB) combined with Zinc (Zn) on Testicular function of Adult Male Sprague dawley Rats.
Materials and methods: Twenty-four adult males praguedawley Rats weighing 180-200g, aged 10-12 weeks, were randomized into four groups (A,B,C,D) of six rats each (n = 6) and were given 2mls of distilled water; 500 mg/kg of SB ; 500 mg/kg of SB and 0.5 mg/kg Znsulfate; 500 mg/kg of SB and 1mg/kg of Zn. The administration was done daily via gastric gavage for 28 days. Parameterstested include: testicular histology, sperm parameters, haematological parameters and Testosterone assay.
Results: There was observed no significant (p > 0.05) increased in testis, epididymal, seminal vesicle, vas deference and prostate gland weight of animals across the group receiving the Seneciobiafrae extract combine with Zn. Testis and serum testosterone levels, sperm count, percentage of sperm viability and motility was higher in the rats administered a combination of Seneciobiafrae extract and Zn compared with controls (p < 0.05). Conversely the percentage of abnormal sperm morphology was decreased (p < 0.05). Histological analysis showed normal spermatogenesis, better association and high density of spermatogenic cells and lumen contains full mature spermatozoa.
Conclusion: Aqueous extract of Seneciobiafrae combined with Zn, potentially enhance testicular function including steroidogenesis and spermatogenesis in male rats.
Viana GS, Bandeira MA, Matos FJ. Analgesic and antiinflammatory effects of chalcones isolated from Myracrodruonurundeuvaallemão. Phytomedicine 2003;
: 189–95.
Okpara JO, Okpala EJ, Ayo JO, Mamman M. Evaluation of the anti-diarrhea activity of AndasoniaDgitata (Boabab) leaves ethanolic extract. Proc. 31st Conf. Nig. Soc. Of Animal Production, held March 12th - 15th, 2006 at Bayero University, Kano, Nigeria. 2006.
Adebooye OC. Solaneciobiafrae (Oliv. &Hiern) C. Jeffrey. [Internet] Record from PROTA4U.Grubben, G.J.H. & Denton, O.A. (Editors). PROTA (Plant Resources of Tropical Africa / Ressourcesvégétales de l’Afriquetropicale), Wageningen, Netherlands.< https://www.prota4u.org/database/protav8.asp?g=pe&p=Solanecio+biafrae+(Oliv.+&+Hiern)+C.Jeffrey>; (accessed on 10/4/2018)
Nupo SS, Onigbogi IO, Akinlotan JV, Ilori OA. Effect of different processing methods on the nutrients and antinutrient composition of seneciobiafrae, crassocephalumcrepidiodes and solanumnigrum consumed in south west Nigeria. American Journal of Food and Nutrition 2013; 3: 147–54.
Adekomi DA, Tijani AA, Adeniyi TD, Olajide JO. Some of the Effects of Aqueous Leaf Extracts of Cnidoscolusaconitifolius (Euphorbiaceae) on the Morphology and Histology of the Kidney and Liver of Sprague Dawley Rat. The Tropical Journal Health Sciences 2011; 18: 9–15.
Akindahunsi AA, Salawu SO. Antioxidant Indices of some Tropical Vegetables. Trop Sci 2006; 45: 33–5.
Telefo PB, Moundipa PF, Tchouanguep FM. Oestrogenicity and effect on hepatic metabolism of the aqueous extract of the leaf mixture of Aloe buettneri, Diclipteraverticillata, Hibiscus macranthus and Justiciainsularis. Fitoterapia 2002; 73: 472–78.
8. Cherdshewasart W, Kitsamai Y, Malaivijitnond S. Evaluation of the estrogenic activity of the Wild Puerariamirifica by vaginal cornification assay. J Reprod Dev 2007; 53: 385–93.
Ganguly M, Borthakur M, Devi N, Mahantam R. Antifertility activity of the methanolic leaf extract of Cissampelospareira in female albino mice. Journal of Ethnopharmacology 2007; 111: 688–91.
Frederickson CJ. Neurobiology of zinc and zinc containing neurons.Int Rev Neurobiol1989; 31:145–38.
Powell SR.The antioxidant properties of zinc. J Nutr 2000; 130: 1447S–54S.
Ozturk A, Baltaci AK, Mogulkoc R, Oztekin E, Sivrikaya A, Kurtogh E, et al. Effects of zinc deficiency and supplementation on malondialdehyde and glutathione levels in blood and tissue of rats performing swimming exercise. Biol Trace ElemRes 2003; 94: 157–66.
Ozdemir G, Inanc F. Zinc may protect remote ocular injury caused by intestinal ischemia reperfusion in rats. Tohoku J Exp Med 2005; 206: 247–51.
Sato M, Bremner I. Oxygen free radicals and metalothionein. Free Radical Biol Med 1993; 14: 325–37.
Özaslan M, Kılıç IH, Aytekin T, Güldür ME, Bozkurt AI. Investigation of Antioxidant Effect of Zinc Biochemically and Histopathologically in Rats.Biotechnology & Biotechnological Equipment 2005; 19: 136–43.
Zhou Z, Wang L, Song Z, Saari JT, McClain CJ, Kang YJ. Zinc Supplementation Prevents Alcoholic Liver Injury in Mice through Attenuation of Oxidative Stress. Am JPathol 2005; 166: 1681–90.
Oteiza PI, Adonaylo VN, Keen CL. Cadmium-induced testes oxidative damage in rats can be influenced by dietary zinc intake. Toxicology 1999 10; 137: 13–22.
Shaheen AA, El-Fattah AA. Effect of dietary zinc on lipid peroxidation, glutathione, protein levels and superoxide dismutase activity in rat tissues.Int J Biochem Cell Biol 1995; 27: 89–95.
Muhammed AO, Adekomi DA, Tijanic AA. Effects of aqueous crude leaf extract of seneciobiafrae on the histology of the frontal cortex, kidney, liver and testis of male spraguedawley rats. Scientific Journal of Biological Sciences 2012; 1:13–18.
Iweala EJ, Okeke CU. Comparative study of the hypoglycemic and biochemical effects of Catharanthusroseus (Linn) g. apocynaceae (Madagascar periwinkle) and chlorpropamide (diabenese) on alloxan-induced diabetic rats. Biokemistri 2005; 17:149–56.
Khan MRI, Islam MA, Hossain MS, Asadujjaman M, Wahed MII, Rahman BM, et al. Antidiabetic Effects of the Different Fractions of Aqueous Extracts of Ocimum sanctum in Normal and Alloxan Induced Diabetic Rats. J Sci Res 2010; 2: 158–68.
Soni A, Sosa S. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. Journal of Pharmacognosy and Phytochemistry 2013; 2:22–9.
Grindberg T, Williams K, Vitamin C quantification using reversed-phase ionpairing HPLC, Concordia Coll J Anal Chem 2010;1:19–23.
Akubugwo IE, ObasiNA,Chinyere GC, Ugbogu AE. Mineral and phytochemical contents in leaves of Amaranthushybridus L. and Solanumnigrum L. subjected to different processing methods. African Journal of Biochemistry Research 2008; 2: 040–0 4.
Fadda WA, Mansour FK, El-Habiby MM, El-Safty FA. Histological and immunohisto-chemical study on the effect of lead on development of testis in albino rat and role of zinc supplementation. Minoufia Medical Journal 2008; 21:77–90.
National Institutes of Health. Guide for the Care and Use of Laboratory Animals. DHEW Publication (NIH), Bethesda, USA, 1985.
Ibegbu AO, Ayuba M, Animoku AA, Brosu D, Adamu SA, Akpulu P, et al. Effect of Ascorbic acid on Mercury-induced changes on the liver in Adult Wistar Rats.IOSR Journal of Dental and Medical Sciences 2014; 13: 10–16.
Biswas NM, Sengupta R, Chatopadhyay GR, Choudhury A, Sarkar M. Effect of ethanol on cadmium-induced testicular toxicity in male rats. ReprodToxicol 2001; 15: 699– 04.
Freund M, Carol B. Factor's affecting haemocytometer counts of sperm concentration in human semen. J ReprodFertil 1964; 8: 149–55.
Hoppe PC, Pitts S. Fertilization in vitro and development of mouse ova. BiolReprod 1973; 8: 420–6.
Akang EN , Oremosu AA, Osinubib AA, Dosumu OO, Kusemiju TO, Adelakun SA, et al. Histomorphometric studies of the effects of Telfairiaoccidentalisonalcohol-induced gonado-toxicity in male rats. Toxicol Rep 2015; 2: 968–75.
Zinaman MJ, Brown CC, Selevan SG, Clegg ED. Semen quality and human fertility: A prospective study with healthy couples. J Androl 2000; 21: 145–53.
Eliasson R. Basic Semen Analysis. In: Current Topics in Andrology, Matson, P. (Ed.). Ladybrook Publishing, Perth, WA, 2003: 35–89.
Menkveld R, Holleboom CA, Rhemrev JP. Measurement and significance of sperm morphology. Asian J Androl 2011; 13: 59–68.
Duke J. The Green Pharmacy: The ultimate compendium of natural remedies from the world’s foremost authority on healing herbs. Rodale press.1998; 248–81.
Mosher WD, Pratt WF. Fecundity and infertility in the United States: Incidence and trends. J FertilSteril 1991; 56:192–3.
Allan DJ, Harmon BV, Roberts SA. Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat. Cell Prolif 1992; 25:241–50.
Tiwari A, Jadon RS, Tiwari P, Nayak S. Phytochemical Investigations of Crown of Solanummelongena fruit. International Journal of Phytomedicine 2009; 1: 9–11.
Saalu LC, Oluyemi KA, Omotuyi IO. a-Tocopherol (vitamin E) attenuates the testicular toxicity associated with experimental cryptorchidism in rats. African Journal of Biotechnology 2007; 12: 1373–7.
Kellokumpu S, Rajaniemi H. Effect of zinc on the uptake of human chorionic gonadotropin (hCG) in rat testis and testosterone response in vivo. BiolReprod 1981; 24:298– 05.
Ruwanpura SM, McLachlan RI, Meachem SJ. Hormonal regulation of male germ cell development. J Endocrinol 2010; 205:117–31.
Hesketh JE. Effects of dietary zinc deficiency on Leydig cell ultrastructure in the boar. J Comp Pathol 1982; 92:239–47.
Oteiza PI, Clegg MS, Keen CL. Short-term zinc deficiency affects nuclear factor-B nuclear binding activity in rat testes. J Nutr 2001; 131:21–6.
Kundu TK, Rao MRS. Zinc dependent recognition of a human CpG island sequence by the mammalian spermatidal protein TP2. Biochemistry 1996; 35:15626–32.
Pradeepa MM. Chromatin remodeling during mammalian spermatogenesis: role of testis specific histone variants and transition proteins. SocReprodFertilSuppl 2007; 63:1–10.
Saleem TKM, Azeem AK, Dilip C, Sanker C, Prasanth NV, Duraisami R. Anti-inflammatory activity of the leaf extracts of Gendarussa Vulgaris Nees. Asian Pac J Trop Biomed 2011; 2: 147–9.
Sies H, Schewe T, Heiss C, Kelm M. Cocoa polyphenols and inflammatory mediators. Am J ClinNutr 2005; 81: 304S – 12S.
Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2010; 2010. pii: 686137.
Eskenazi B, Kidd SA, Marks AR, Sloter E, Block G, Wyrobek AJ. Antioxidantintake is associated with semen quality in healthy men. Human Reproduction 2005; 20:1006–12.
Geva E, Bartoov B, Zabludovsky N, Lessing JB, Lerner-Geva L, Amit A. The effect of antioxidant treatment on human spermatozoa and fertilization rate in an invitro fertilization program.FertilSteril 1996; 66:430–4.
Comhaire FH, Christophe AB, Zalata AA, Dhooge WS, Mahmoud AM, Depuydt CE. The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men. Prostaglandins LeukotEssent Fatty Acids 2000; 63: 159–65.
Kayode AAA, Kayode OT. Some medicinal values of Telfairiaoccidentalis: a review. American Journal of Biochemistry and Molecular Biology 2011; 1:30–8.
Rodrigues HG, Diniz YS, Faine LA, Galhardi CM, Burneiko RC, Almeida JA, et al. Antoxidant effect of Saponin: potential action of a soybean flavonoid on glucose tolerance and risk factors for atherosclerosis. Int J food SciNutr 2005; 56: 79–85.
Saleh RA, Agarwal A. Oxidative stress and male infertility: from research bench to clinical practice. J Androl 2002; 23: 737–52.
Fasuyi AO. Nutritional potentials of some tropical vegetable leaf meals Chemical characterization and functional properties. African Journal of Biotechnology 2006; 5: 49–53.
Bucak MN, Atessahin A, Yuce A. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze–thawing process. Small Ruminant Research 2008; 75 :128–34.
Mohammadi S, Movahedin M, Mowla SJ. Antioxidant effects of selenium on sperm parameters and testicular structure in young and aged mice. Journal of reproduction & Infertility 2008; 9: 229–37.
Yousef MI, Abdallah GA, Kamel KI. Effect of ascorbic acid and vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim Reprod Sci 2003; 76: 99–11.
Sonmez M, Turk G, Yuce A. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 2005; 63: 2063–72.
Narayana K, Prashanthi N, Nayanatar A, Kumar HHC, Abhilash K, Bairy KL. Effects of methyl parathion (o,o-dimethyl o-4-nitropheny phosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutat Res 2005; 588: 28–34.
Shittu LAJ, Bankole MA, Oguntola JA, Ajala O, Shittu RK, Ogundipe OA, et al. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male Sprague Dawley rat. Scientific Research and Essay 2007; 2: 319–24.
Cody V, Middleton E, Harbone JB. Plant Flavonoids in Biology and Medicine (eds.): Biochemical, Pharmacological and Structural-Activity Relationships, Alan Liss, New York. 1986:22–25.
Harborne JB, Williams CA. Advances in flavonoids research since 1992. Phytochemistry 2000; 55: 481–504.
| Files | ||
| Issue | Vol 12, No 1 (March 2018) | |
| Section | Original Articles | |
| Keywords | ||
| Seneciobiafrae Zinc Lumen Spermatozoa Testosterone | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
