Concurrent Lead and Noise Exposure Effects on Testicular Tissue of Rat: An Experimental Study
-
Batol Masruri
,
Iraj Alimohammadi
,
Mohammad Reza Abyaz
,
Nargess Moghadasi
,
Parvaneh Yekzamani
,
Baharak Mohammadzadeh Asl
,
Nooshin Asadmasjedi
,
Azadeh Ashtarinezhad
Abstract
Objective: Environmental stressors such as physical and chemical factors can have a destructive testicular function. The present study aimed to determine the effect of concomitant exposure to lead and noise on testicular tissue in male rats.
Materials and methods: Twenty male Wistar rats (250-300 g, 12-13wk) were divided into four groups (n=5/each): 1) Control group, 2) Lead group treated with 4 mg/kg lead acetate by gavage for 30 days,
3) Noise group exposed to 4 kHz octave band at 105 dBA for eight hrs./ day for 30 days, and 4) the exposed group to lead plus noise concurrently. The testes' weight was measured, and testes abnormalities were assessed after staining with Hematoxylin and Eosin.
Results: The results showed that the weight of testes in experimental groups was significantly decreased compared with the control group (p<0.0001). Also indicated edema, degeneration and necrotic cell debris in the lumen, congestion and atrophy of seminiferous tubule in rat testes tissue due to sub-acute exposure to lead and noise.
Conclusion: Exposure to 105 dB and lead can cause damage to the seminiferous tubules, intubation edema, and testicular weight loss compared to control. We also found that simultaneous exposure to noise and lead could have more detrimental effects on testicular histology and weight than others.
1. Singh A, Sharma R. Effects of stress on epididymis in albino rat. Int J Med Clin Res 2011; 2: 20-1.
2. Farzadinia P, Bigdeli M, Akbarzadeh S, Mohammadi M, Daneshi A, Bargahi A. Effect of noise pollution on testicular tissue and hormonal assessment in rat. Andrologia 2016; 48: 869-73.
3. Camille Melón L, Maguire J. GABAergic regulation of the HPA and HPG axes and the impact of stress on reproductive function. J Steroid Biochem Mol Biol 2016; 160: 196-203.
4. Nadri F, Khavanin A, Mazaheri Z, Khajehnasiri F. The effect of noise stress on adult male rat sperm parameters and the protective effect of hydroalcoholic Cinnamomum verum extract: an experimental study. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ) 2018; 20: e69779.
5. Golmohammadi R, Giahi O, Aliabadi M, Darvishi E. An intervention for noise control of blast furnace in steel industry. J Res Health Sci 2014; 14: 287-90.
6. Swami CG, Ramanathan J, Charan Jeganath C. Noise exposure effect on testicular histology, morphology and on male steroidogenic hormone. Malays J Med Sci 2007; 14:28-35.
7. Jalali M, Hemadi M, Saki G, Sarkaki A. Study of spermatogenesis fetal testis exposed noise stress during and after natal period in rat. Pak J Biol Sci 2013; 16: 1010-5.
8. El-Magd MA, Kahilo KA, Nasr NE, Kamal T, Shukry M, Saleh AA. A potential mechanism associated with lead-induced testicular toxicity in rats. Andrologia 2017; 49.
9. Apaydin FG, Kalender S, Bas H, Demir F, Kalender Y. Lead nitrate induced testicular toxicity in diabetic and non-diabetic rats: protective role of sodium selenite. Brazilian Archives of Biology and Technology 2015; 58: 68-74.
10. Ahmed YF, Eldebaky HAA, Mahmoud KG, Nawito M. Effects of lead exposure on DNA damage and apoptosis in reproductive and vital organs in female rabbits. Global veterinaria 2012; 9: 401-8.
11. Andjelkovic M, Buha Djordjevic A, Antonijevic E, Antonijevic B, Stanic M, Kotur-Stevuljevic J et al. Toxic effect of acute cadmium and lead exposure in rat blood, liver, and kidney. Int J Environ Res Public Health 2019; 16: 274.
12. Ericson B, Landrigan P, Taylor MP, Frostad J, Caravanos J, Keith J, et al. The global burden of lead toxicity attributable to informal used lead-acid battery sites. Ann Glob Health 2016; 82: 686-99.
13. Manoj Kumar V, Henley AK, Nelson CJ, Indumati O, Prabhakara Rao Y, Rajanna S, et al. Protective effect of Allium sativum (garlic) aqueous extract against lead-induced oxidative stress in the rat brain, liver, and kidney. Environ Sci Pollut Res Int 2017; 24: 1544-52.
14. l-Tantawy WH. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J Tradit Complement Med 2015; 6: 327-31.
15. Alimohammadi I, Ashtarinezhad A, Abolghasemi J, Yekzamani P, Masruri B. The Sub-acute Effect of Lead Exposure on the Auditory Brainstem Response in Male Rats. Iranian Journal of Toxicology 2019; 13: 9-13.
16. Fahim MA, Tariq S, Adeghate E. Vitamin E modifies the ultrastructure of testis and epididymis in mice exposed to lead intoxication. Ann Anat 2013; 195: 272-7.
17. Kumar SR, Devi AS. Lead toxicity on male reproductive system and its mechanism: a review. Research Journal of Pharmacy and Technology 2018; 11: 1228-32.
18. Halliwell B. Free radicals and antioxidants: updating a personal view. Nutr Rev 2012; 70: 257-65.
19. Masruri B, Ashtarinezhad A, Yekzamani P. Data on the effect of lead concomitant noise on oxidative stress in rats. Data Brief 2018; 18: 1117-21.
20. Liu S, Zhang K, Wu S, Ji X, Li N, Liu R, et al. Lead-induced hearing loss in rats and the protective effect of copper. Biological trace element research 2011; 144: 1112-9.
21. Haghighat M, Allameh A, Fereidan M, Khavanin A, Ghasemi Z. Effects of concomitant exposure to styrene and intense noise on rats’ whole lung tissues. Biochemical and histopathological studies. Drug Chem Toxicol 2022; 45: 120-6.
22. Shaha C, Tripathi R, Mishra DP. Male germ cell apoptosis: regulation and biology. Philos Trans R Soc Lond B Biol Sci 2010; 365: 1501-15.
23. Cao Y, Wang D, Li Q, Deng H, Shen J, Zheng G, et al. Rat testis damage caused by lead sulfide nanoparticles after oral exposure. J Nanosci Nanotechnol 2016; 16: 2378-83.
24. World Health Organization. Regional Office for Europe. Burden of disease from environmental noise: quantification of healthy life years lost in Europe. World Health Organization. Regional Office for Europe.2011
25. Pramanik P, Biswas S. Traffic noise: a silent killer of male gamete of albino rats. Al Ameen J Med Sci 2012; 5: 82-9.
26. Chen Cárdenas S, Mayer N, Romanini M, Rolando A, Liaudat A, Brun N, et al. Reproductive response in offspring male rats exposed to prenatal stress and to early postnatal stimulation. International Journal of Morphology 2013; 31: 754-64.
27. Garu U, Sharma R, Barber I. Effect of lead toxicity on developing testis of mice. International Journal of Pharmaceutical Sciences and Research 2011; 2: 2403.
28. Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011; 2011: 457327.
29. Kasperczyk A, Dobrakowski M, Czuba ZP, Horak S, Kasperczyk S. Environmental exposure to lead induces oxidative stress and modulates the function of the antioxidant defense system and the immune system in the semen of males with normal semen profile. Toxicol Appl Pharmacol 2015; 284: 339-44.
30. Ruff HA, Markowitz ME, Bijur PE, Rosen JF. Relationships among blood lead levels, iron deficiency, and cognitive development in two-year-old children. Environ Health Perspect 1996; 104: 180-5.
31. Aksu D, Sağlam Y, Yildirim S, Aksu T. Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats. Cell Mol Biol (Noisy-le-grand) 2017; 63: 33-42.
32. Mustafa HN. Potential alleviation of Chlorella vulgaris and Zingiber officinale on lead-induced testicular toxicity: an ultrastructural study. Folia Biol (Krakow) 2015; 63: 269-78.
33. Babisch W. Transportation noise and cardiovascular risk: updated review and synthesis of epidemiological studies indicate that the evidence has increased. Noise Health 2006; 8: 1-9.
34. Chehreie S, Rabzia A, Farhadi-mesterkhani M. Maternal Water Deprivation Affects the Spermatogenesis of the Offspring Rats. International Journal of Morphology 2013; 31: 156-61.
35. Ma L, Liu J, Li N, Wang J, Duan Y, Yan J, et al. Oxidative stress in the brain of mice caused by translocated nanoparticulate TiO2 delivered to the abdominal cavity. Biomaterials 2010; 31: 99-105.
36. Hasanein P, Fazeli F, Parviz M, Roghani M. Ferulic acid prevents lead‐induced testicular oxidative stress and suppressed spermatogenesis in rats. Andrologia 2018; 50: e12798.
37. Ali S, Al-Dervi KH, Al Monsour NAA. Testicular toxic effect of lead acetate on adult male rats and the potential protective role of alcoholic extract of ginseng (histological, histomorphometrical and physiological). Scientific Journal of Medical Research 2018; 2: 87-92.
38. Modaresi M, Messripour M, Rajaei RA. Effect of cinnamon extract on the number of spermatocyte and spermatozoa cells in mice. Iranian Journal of Medicinal and Aromatic Plants 2010; 26: 83-90.
39. Dorostghoal M, Seyyednejad SM, Jabari A. Protective effects of F umaria parviflora L. on lead‐induced testicular toxicity in male rats. Andrologia 2014; 46: 437-46.
40. Saxena DK, Srivastava RS, Lal B, Chandra SV. The effect of lead exposure on the testis of growing rats. Exp Pathol 1987; 31: 249-52.
41. Moniem AEA, Dkhil MA, Al-Quraishy S. Protective role of flaxseed oil against lead acetate induced oxidative stress in testes of adult rats. African Journal of Biotechnology 2010; 9: 7216-23.
42. Graça A, Ramalho-Santos J, de Lourdes Pereira M. Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl 2004; 6: 237-41.
43. Pirami H, Khavanin A, Mazaheri Z, Nadri F. The protective effect of cinnamon hydroalcoholic extract on testicular tissue changes and fertility capacity in male rats exposed to noise stress. Journal of Birjand University of Medical Sciences 2018; 25: 21-30.
2. Farzadinia P, Bigdeli M, Akbarzadeh S, Mohammadi M, Daneshi A, Bargahi A. Effect of noise pollution on testicular tissue and hormonal assessment in rat. Andrologia 2016; 48: 869-73.
3. Camille Melón L, Maguire J. GABAergic regulation of the HPA and HPG axes and the impact of stress on reproductive function. J Steroid Biochem Mol Biol 2016; 160: 196-203.
4. Nadri F, Khavanin A, Mazaheri Z, Khajehnasiri F. The effect of noise stress on adult male rat sperm parameters and the protective effect of hydroalcoholic Cinnamomum verum extract: an experimental study. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ) 2018; 20: e69779.
5. Golmohammadi R, Giahi O, Aliabadi M, Darvishi E. An intervention for noise control of blast furnace in steel industry. J Res Health Sci 2014; 14: 287-90.
6. Swami CG, Ramanathan J, Charan Jeganath C. Noise exposure effect on testicular histology, morphology and on male steroidogenic hormone. Malays J Med Sci 2007; 14:28-35.
7. Jalali M, Hemadi M, Saki G, Sarkaki A. Study of spermatogenesis fetal testis exposed noise stress during and after natal period in rat. Pak J Biol Sci 2013; 16: 1010-5.
8. El-Magd MA, Kahilo KA, Nasr NE, Kamal T, Shukry M, Saleh AA. A potential mechanism associated with lead-induced testicular toxicity in rats. Andrologia 2017; 49.
9. Apaydin FG, Kalender S, Bas H, Demir F, Kalender Y. Lead nitrate induced testicular toxicity in diabetic and non-diabetic rats: protective role of sodium selenite. Brazilian Archives of Biology and Technology 2015; 58: 68-74.
10. Ahmed YF, Eldebaky HAA, Mahmoud KG, Nawito M. Effects of lead exposure on DNA damage and apoptosis in reproductive and vital organs in female rabbits. Global veterinaria 2012; 9: 401-8.
11. Andjelkovic M, Buha Djordjevic A, Antonijevic E, Antonijevic B, Stanic M, Kotur-Stevuljevic J et al. Toxic effect of acute cadmium and lead exposure in rat blood, liver, and kidney. Int J Environ Res Public Health 2019; 16: 274.
12. Ericson B, Landrigan P, Taylor MP, Frostad J, Caravanos J, Keith J, et al. The global burden of lead toxicity attributable to informal used lead-acid battery sites. Ann Glob Health 2016; 82: 686-99.
13. Manoj Kumar V, Henley AK, Nelson CJ, Indumati O, Prabhakara Rao Y, Rajanna S, et al. Protective effect of Allium sativum (garlic) aqueous extract against lead-induced oxidative stress in the rat brain, liver, and kidney. Environ Sci Pollut Res Int 2017; 24: 1544-52.
14. l-Tantawy WH. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J Tradit Complement Med 2015; 6: 327-31.
15. Alimohammadi I, Ashtarinezhad A, Abolghasemi J, Yekzamani P, Masruri B. The Sub-acute Effect of Lead Exposure on the Auditory Brainstem Response in Male Rats. Iranian Journal of Toxicology 2019; 13: 9-13.
16. Fahim MA, Tariq S, Adeghate E. Vitamin E modifies the ultrastructure of testis and epididymis in mice exposed to lead intoxication. Ann Anat 2013; 195: 272-7.
17. Kumar SR, Devi AS. Lead toxicity on male reproductive system and its mechanism: a review. Research Journal of Pharmacy and Technology 2018; 11: 1228-32.
18. Halliwell B. Free radicals and antioxidants: updating a personal view. Nutr Rev 2012; 70: 257-65.
19. Masruri B, Ashtarinezhad A, Yekzamani P. Data on the effect of lead concomitant noise on oxidative stress in rats. Data Brief 2018; 18: 1117-21.
20. Liu S, Zhang K, Wu S, Ji X, Li N, Liu R, et al. Lead-induced hearing loss in rats and the protective effect of copper. Biological trace element research 2011; 144: 1112-9.
21. Haghighat M, Allameh A, Fereidan M, Khavanin A, Ghasemi Z. Effects of concomitant exposure to styrene and intense noise on rats’ whole lung tissues. Biochemical and histopathological studies. Drug Chem Toxicol 2022; 45: 120-6.
22. Shaha C, Tripathi R, Mishra DP. Male germ cell apoptosis: regulation and biology. Philos Trans R Soc Lond B Biol Sci 2010; 365: 1501-15.
23. Cao Y, Wang D, Li Q, Deng H, Shen J, Zheng G, et al. Rat testis damage caused by lead sulfide nanoparticles after oral exposure. J Nanosci Nanotechnol 2016; 16: 2378-83.
24. World Health Organization. Regional Office for Europe. Burden of disease from environmental noise: quantification of healthy life years lost in Europe. World Health Organization. Regional Office for Europe.2011
25. Pramanik P, Biswas S. Traffic noise: a silent killer of male gamete of albino rats. Al Ameen J Med Sci 2012; 5: 82-9.
26. Chen Cárdenas S, Mayer N, Romanini M, Rolando A, Liaudat A, Brun N, et al. Reproductive response in offspring male rats exposed to prenatal stress and to early postnatal stimulation. International Journal of Morphology 2013; 31: 754-64.
27. Garu U, Sharma R, Barber I. Effect of lead toxicity on developing testis of mice. International Journal of Pharmaceutical Sciences and Research 2011; 2: 2403.
28. Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011; 2011: 457327.
29. Kasperczyk A, Dobrakowski M, Czuba ZP, Horak S, Kasperczyk S. Environmental exposure to lead induces oxidative stress and modulates the function of the antioxidant defense system and the immune system in the semen of males with normal semen profile. Toxicol Appl Pharmacol 2015; 284: 339-44.
30. Ruff HA, Markowitz ME, Bijur PE, Rosen JF. Relationships among blood lead levels, iron deficiency, and cognitive development in two-year-old children. Environ Health Perspect 1996; 104: 180-5.
31. Aksu D, Sağlam Y, Yildirim S, Aksu T. Effect of pomegranate (Punica granatum L.) juice on kidney, liver, heart and testis histopathological changes, and the tissues lipid peroxidation and antioxidant status in lead acetate-treated rats. Cell Mol Biol (Noisy-le-grand) 2017; 63: 33-42.
32. Mustafa HN. Potential alleviation of Chlorella vulgaris and Zingiber officinale on lead-induced testicular toxicity: an ultrastructural study. Folia Biol (Krakow) 2015; 63: 269-78.
33. Babisch W. Transportation noise and cardiovascular risk: updated review and synthesis of epidemiological studies indicate that the evidence has increased. Noise Health 2006; 8: 1-9.
34. Chehreie S, Rabzia A, Farhadi-mesterkhani M. Maternal Water Deprivation Affects the Spermatogenesis of the Offspring Rats. International Journal of Morphology 2013; 31: 156-61.
35. Ma L, Liu J, Li N, Wang J, Duan Y, Yan J, et al. Oxidative stress in the brain of mice caused by translocated nanoparticulate TiO2 delivered to the abdominal cavity. Biomaterials 2010; 31: 99-105.
36. Hasanein P, Fazeli F, Parviz M, Roghani M. Ferulic acid prevents lead‐induced testicular oxidative stress and suppressed spermatogenesis in rats. Andrologia 2018; 50: e12798.
37. Ali S, Al-Dervi KH, Al Monsour NAA. Testicular toxic effect of lead acetate on adult male rats and the potential protective role of alcoholic extract of ginseng (histological, histomorphometrical and physiological). Scientific Journal of Medical Research 2018; 2: 87-92.
38. Modaresi M, Messripour M, Rajaei RA. Effect of cinnamon extract on the number of spermatocyte and spermatozoa cells in mice. Iranian Journal of Medicinal and Aromatic Plants 2010; 26: 83-90.
39. Dorostghoal M, Seyyednejad SM, Jabari A. Protective effects of F umaria parviflora L. on lead‐induced testicular toxicity in male rats. Andrologia 2014; 46: 437-46.
40. Saxena DK, Srivastava RS, Lal B, Chandra SV. The effect of lead exposure on the testis of growing rats. Exp Pathol 1987; 31: 249-52.
41. Moniem AEA, Dkhil MA, Al-Quraishy S. Protective role of flaxseed oil against lead acetate induced oxidative stress in testes of adult rats. African Journal of Biotechnology 2010; 9: 7216-23.
42. Graça A, Ramalho-Santos J, de Lourdes Pereira M. Effect of lead chloride on spermatogenesis and sperm parameters in mice. Asian J Androl 2004; 6: 237-41.
43. Pirami H, Khavanin A, Mazaheri Z, Nadri F. The protective effect of cinnamon hydroalcoholic extract on testicular tissue changes and fertility capacity in male rats exposed to noise stress. Journal of Birjand University of Medical Sciences 2018; 25: 21-30.
| Files | ||
| Issue | Vol 16, No 2 (June 2022) | |
| Section | Original Articles | |
| DOI | https://doi.org/10.18502/jfrh.v16i2.9483 | |
| Keywords | ||
| Lead acetate Noise Testis Rat Infertility | ||
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How to Cite
1.
Masruri B, Alimohammadi I, Abyaz MR, Moghadasi N, Yekzamani P, Mohammadzadeh Asl B, Asadmasjedi N, Ashtarinezhad A. Concurrent Lead and Noise Exposure Effects on Testicular Tissue of Rat: An Experimental Study. J Family Reprod Health. 2022;16(2):132-138.
