Effect of Vitex Agnus-Castus Ethanolic Extract on Sex Hormones in Streptozotocin-Induced Diabetic Rats

  • Fatemeh Soleymanzadeh ORCID Department of Biology, Faculty of Basic Science, Hamedan Islamic Azad University, Hamadan, Iran
  • Minoo Mahmoodi Mail Department of Biology, Faculty of Basic Science, Hamedan Islamic Azad University, Hamadan, Iran
  • Siamak Shahidi ORCID Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Keywords:
Vitex Agnus-Castus, Diabetes, Streptozotocin, Sex Hormones

Abstract

Objective: Diabetes mellitus is recognized as one of the serious global health problems. There are evidences regarding the high prevalence of sexual dysfunction in diabetic patients. Experimental studies revealed a positive effect of Vitex agnus-castus (Vitex), on sexual function and behaviors. In this research, the effect of Vitex on sexual hormones in streptozotocin-(STZ) induced diabetic rats was investigated.

Materials and methods: A Thirty adult female Wistar rats were divided into five groups. 1-control group (non-diabetic), 2- diabetic group (received normal saline) and three induced diabetic groups treated with different doses (400, 200 and 100 mg/kg) of Vitex. Treatment groups received Vitex fruit extract by gavage for 7 days. The levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone and estrogen in serum were measured.
Results: Levels of LH, FSH, estrogen and progesterone and average body weight was lower in diabetic group compared to control group (p <0.010). Animals received high dose of Vitex fruit extract (400mg/kg) had significantly higher levels of serum LH, FSH, estrogen and progesterone compared to diabetic group (p < 0.010). In animals receiving minimum dose (100mg/kg) of Vitex, no difference was observed compared to diabetic group (p > 0.010).
Conclusion: It can be concluded that Vitex fruit extract probably has regulatory effect on diabetes-induced change in the levels of sex hormones in female rats. Vitex fruit extract can improve serum levels of sex hormones in an animal model of STZ-induced diabetes.

References

1. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The Role of Oxidative Stress and Antioxidants in Diabetic Complications. Sultan Qaboos Uni Med J 2012; 12: 5-18.
2. Maiorino MI, Bellastella G, Esposito K. Diabetes and sexual dysfunction: current perspectives. Diabetes Metab Syndr Obes 2014; 7: 95–105.
3. Rani A, Sharma A. The genus Vitex: A review. Pharmacogn Rev 2013; 7: 188–98.
4. Niroumand MC, Heydarpour F, Farzaei MH. Pharmacological and therapeutic effects of Vitex
agnus-castus L.: A review. Phcog Rev 2018; 12: 103-14.
5. Ahangarpour A, Oroojan AA, Khorsandi L, Najimi SA. Pancreatic protective and hypoglycemic effects of Vitex agnus-castus L. fruit hydroalcoholic extract in
D-galactose-induced aging mouse model. Res Pharm Sci 2017; 12: 137–43.
6. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001; 50: 537-46.
7. Bellamkonda R, Rasineni K, Singareddy SR, Kasetti RB, Pasurla R, Chippada AR, et al. Antihyperglycemic and antioxidant activities of alcoholic extract of Commiphora mukul gum resin in streptozotocin induced diabetic rats. Pathophysiology 2011; 18: 255-61.
8. Baynes JW, Thorpe SR. Role of oxidative stress in diabetic complications: A new perspective on an old paradigm. Diabetes 1999; 48: 1–9.
9. Seethalakshmi L, Menon M, Diamond D. The effect of streptozotocin-induced diabetes on the neuroendocrine-male reproductive tract axis of the adult rat. J Urol 1987; 138: 190-4.
10. Bucholtz DC, Chiesa A, Pappano WN, Nagatani SH,
Tsukamura H, Maeda K, et al. Regulation of Pulsatile Luteinizing Hormone Secretion by Insulin in the Diabetic Male Lamb. Biol Reprod 2000; 62: 1248–55.
11. Kim NN, Stankovic M, Cushman TT, Goldstein I, Munarriz R, Traish AM. Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers. BMC Physiol 2006; 6: 4.
12. Seethalakshmi L, Menon M, Diamond D. The effect of streptozotocin-induced diabetes on the neuroendocrine-male reproductive tract axis of the adult rat. J Urol 1987; 138; 190-4.
13. Tanaka T, Nagatani S, Bucholtz DC, Ohkura S, Tsukamura H, Maeda K, et al. Central action of insulin regulates pulsatile luteinizing hormone secretion in the diabetic sheep model. Biol. Reprod 2000; 62: 1256-61.
14. Jelodar G, Askari K. Effect of Vitex agnus-castus fruits hydroalcoholic extract on sex hormones in rat with induced polycystic ovary syndrome (PCOS). Physiol Pharmacol 2012; 16: 62-9.
15. Ibrahim NA, Shalaby AS, Farag RS, Elbaroty GS, Nofal SM,Hassan EM. Gynecological efficacy and chemical investigation of Vitex agnus-castus L. fruits growing in Egypt. Nat Prod Res 2008; 22: 537-46.
16. Shaw S, Wyatt K, Campbell J, Ernst E, Thompson-Coon J.Vitex agnus castus for premenstrual syndrome. Cochrane Database Syst Rev 2018; 2018: CD004632.
17. Bergmann J, Luft B, Boehmann S, Runnebaum B, Gerhard I. The efficacy of the complex medication Phyto-Hypophyson L in female, hormone-related sterility. A randomized, placebo-controlled clinical double-blind study. Forsch Komplementarmed Klass Naturheilkd 2000; 7: 190-9.
18. Milewicz A, Gejdel E, Sworen H, Sienkiewicz K, Jedrzejak J, Teucher T, et al. vitex agnus castus extracted in the treatment of luteal phase defect due to latent hyperprolactinemia. Arzneimittelforschung 1993; 43: 752-6.
19. Jarry H, Spengler B, Porzel A, Schmidt J, Wuttke W, Christoffel V, et al. Evidence for estrogen receptor beta-selective activity of Vitex agnus-castus and isolated flavones. Planta Med 2003; 69: 945-7.
20. Liu J, Burdette JE, Sun Y, Deng S, Schlecht SM, Zheng W, et al. Isolation of linoleic acid as an estrogenic compound from the fruits of Vitex agnus-castus L (chaste-berry) Phytomedicine, 2004; 11: 18-23.
Published
2020-10-18
How to Cite
1.
Soleymanzadeh F, Mahmoodi M, Shahidi S. Effect of Vitex Agnus-Castus Ethanolic Extract on Sex Hormones in Streptozotocin-Induced Diabetic Rats. J Fam Reprod Health. 14(2):102-105.
Section
Original Articles