Genotypes of John Cunningham (JC) Virus Urinary Excretion in Pregnant and Non-Pregnant Women in Isfahan, Iran

  • Sayyedeh Rahmaneh Atyabi Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
  • Majid Bouzari Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
  • Majid Komijani Department of Biology, Faculty of Science, Arak University, Arak, Iran
Keywords: John Cunningham Virus, Genotypes, Pregnant Women, Isfahan, Urine


Objective: To evaluate presence of different subtypes and genetic variations of JC virus in different geographical areas is a useful tool for reconstructing of the genetic information and understanding of the evolution of the virus and also in tracing of the last and present history of human immigration.Materials and methods: This study aimed to investigate the reactivation of different genotypes of JC virus in kidney and its excretion in the urine of the 50 pregnant and 50 non-pregnant women. Phenol-chloroform method was used to extract DNA. Oligo 7 and MEGA 7 software were used for designing nested PCR specific primers based on vp1 capsid gene, and construction of phylogenetic tree, respectively. Fisher’s exact test was used for statistical analyses.Results: All of the positive samples were sequenced and according to them, genotypes 1 and 3 of the virus were detected for the first time in pregnant and non-pregnant women in Asia. The frequency of genotypes 1 and 3 were 14.28% and 85.71% respectively.Conclusion: For the first time genotype 3 was reported as the frequent genotype in pregnant women in Asia. Confirming these needs more studies particularly with a higher number of cases and full genome sequencing of isolated JCVs.

Author Biography

Majid Bouzari, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
\Professor of Virology Department of Biology


Rasmussen SA, Hayes EB, Jamieson DJ, O'Leary DR. Emerging infections and pregnancy: assessing the impact on the embryo or fetus. ‎Am. J. Med. Genet. 2007;143:2896-2903.

Ferenczy MW, Marshall LJ, Nelson CD, Atwood WJ, Nath A, Khalili K, et al. Molecular biology, epidemiology, and pathogenesis of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain. ‎Clin. Microbiol. Rev. 2012;25:471-506.

Comar M, Zanotta N, Croci E, Murru I, Marci R, Pancaldi C, et al. Association between the JC polyomavirus infection and male infertility. PloS One. 2012;7:e42880-e42880.

Polo C, Perez JL, Mielnichuck A, Fedele CG, Niubo J, Tenorio A. Prevalence and patterns of polyomavirus urinary excretion in immunocompetent adults and children. Clin Microbiol Infect. 2004;10:640-644.

Alosaimi B, Hampson L, He X, Maranga IO, Oliver A, Hampson IN. Increased prevalence of JC polyomavirus in cervical carcinomas from women infected with HIV. J. Med. Virol. 2014;86:672-677.

Hachana M, Amara K, Ziadi S, Gacem RB, Korbi S, Trimeche M. Investigation of human JC and BK polyomaviruses in breast carcinomas. Breast Cancer Res Treat. 2012;133:969-977.

Chang D, Wang M, Ou WC, Lee MS, Ho HN, Tsai RT. Genotypes of human polyomaviruses in urine samples of pregnant women in Taiwan. J. Med. Virol. 1996;48:95-101.

Bendiksen S, Rekvig OP, Van Ghelue M, Moens U. VP1 DNA sequences of JC and BK viruses detected in urine of systemic lupus erythematosus patients reveal no differences from strains expressed in normal individuals. J. Gen. Virol. 2000;81:2625-2633.

Bergallo M, Costa C, Gribaudo G, Tarallo S, Baro S, Ponzi AN, et al. Evaluation of six methods for extraction and purification of viral DNA from urine and serum samples. New Microbiol. 2006;29: 111-119.

Coutlée F, de Ladurantaye M, Tremblay C, Vincelette J, Labrecque L, Roger M. An important proportion of genital samples submitted for Chlamydia trachomatis detection by PCR contain small amounts of cellular DNA as measured by β-globin gene amplification. ‎J. Clin. Microbiol. 2000;38:2512-2515.

Atyabi SR, Bouzari M, Kardi MT. John cunningham (JC) virus genotypes in kidney transplant recipients, rheumatoid arthritis patients and healthy individuals in Isfahan, Iran. J. Med. Virol. 2016, DOI: 10.1002/jmv.24626

Schaffer K, Sheehy N, Coughlan S, Bergin C, Hall WW. JC virus in the Irish population: significant increase of genotype 2 in immunocompromised individuals. ‎J. Neurovirol. 2006;12:39-46.

Hall T. BioEdit. Biological sequence alignment editor for Windows 95/98/NT/2000/XP/7.

Jolla L. Fisher's exact test was performed using GraphPad Prism version 6.01 for Windows, GraphPad Software, California USA, 1994.

S Kumar, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016.

McClure GB, Gardner JS, Williams JT, Copeland CM, Sylvester SK, Garcea RL, et al. Dynamics of pregnancy‐associated polyomavirus urinary excretion: A prospective longitudinal study. J. Med. Virol. 2012;84:1312-1322.

Markowitz RB, Eaton BA, Kubik MF, Latorra D, McGregor JA, Dynan WS. BK Virus and JC Virus Shed during Pregnancy Have Predominantly Archetypal Regulatory Regions. J. Virol. 1991;65(8):4515-4519.

Agostini HT, Ryschkewitsch CF, Mory R, Singer EJ, Stoner GL. JC virus (JCV) genotypes in brain tissue from patients with progressive multifocal leukoencephalopathy (PML) and in urine from controls without PML: increased frequency of JCV type 2 in PML. J. Infect. Dis. 1997;176:1-8.

Baksh FK, Finkelstein SD, Swalsky PA, Stoner GL, Ryschkewitsch CF, Randhawa P. Molecular genotyping of BK and JC viruses in human polyomavirus (ndash ) associated interstitial nephritis after renal transplantation. ‎Am. J. Kidney Dis. 2001;38:354-365.

Frisque RJ, Bream GL, Cannella MT. Human polyomavirus JC virus genome. J. Virol. 1984; 51:458-469.

How to Cite
Atyabi SR, Bouzari M, Komijani M. Genotypes of John Cunningham (JC) Virus Urinary Excretion in Pregnant and Non-Pregnant Women in Isfahan, Iran. JFRH. 12(2):111-6.
Original Articles