Tehran University of Medical Sciences Journal of Family and Reproductive Health 1735-8949 16 3 2022 09 06 212 216 Abolfazl Mazandarani Plasma and Nuclear Fusion Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Tehran, Iran Shervin Goudarzi Plasma and Nuclear Fusion Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization, Tehran, Iran Mina Jafarabadi Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran Elham Azimi Nekoo Jacobi Medical Center/Albert Einstein University, New York, United States 2022 07 06 2022 07 17 Objective: To investigate the effect of cold plasma on Staphylococcus aureus destruction at different treatment times. Materials and methods: Staphylococcus aureus was cultured on 4 plates of LB Agar medium each at 1.5 × 103 CFU / mL (colony-forming unit per milliliter) and one group was selected as the control group and the other 3 groups were treated with plasma for 5, 7 and 10 minutes. They were incubated for 24 hours at 37 °C. Finally, the number of colonies formed was counted. Results: It was shown that treatment with cold atmospheric plasma significantly reduced bacterial colonies and in comparison to the control plate with a colony count of 1.5 × 103 CFU/mL treatment with air plasma for 10 minutes decreased the Pseudomonas colony count to zero. Conclusion: It was observed that the cold atmospheric plasma jet device manufactured in atomic Energy Organization of Iran can significantly kill bacteria in a short time. Increasing the duration of treatment significantly reduces bacterial colonies. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/2125