Tehran University of Medical Sciences Journal of Family and Reproductive Health 1735-8949 8 3 2014 09 15 131 4 Savita Rani Singhal Department of Obstetrics and Gynecology, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. Veena Ghalaut Department of Biochemistry, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. Shashi Lata Department of Obstetrics and Gynecology, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. Himanshu Madaan Department of Biochemistry, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. Veenu Kadian Department of Obstetrics and Gynecology, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. Ashuma Sachdeva Department of Biochemistry, Pt B D Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India. 2015 10 16 Objective: To find shortest and reliable time period of urine collection for determination of proteinuria. Materials and methods: It is a prospective study carried out on 125 pregnant women with preeclampsia after 20 weeks of gestation having urine albumin >1 using dipstick test. Urine was collected in five different time intervals in colors labeled containers with the assistance of nursing staff; the total collection time was 24 hours. Total urine protein of two-hour, four-hour, eight-hour, 12-hour and 24-hour urine was measured and compared with 24-hour collection. Data was analyzed using the Pearson correlation coefficient. Results: There was significant correlation (p value < 0.01) in two, four, eight and 12-hour urine protein with 24-urine protein, with correlation coefficient of 0.97, 0.97, 0.96 and 0.97, respectively. When a cut off value of 25 mg, 50 mg. 100 mg, and 150 mg for urine protein were used for 2-hour, 4-hours, 8-hour and 12-hour urine collection, a sensitivity of 92.45%, 95.28%, 91.51%, and 96.23% and a specificity of 68.42%, 94.74%, 84.21% and 84.21% were obtained, respectively. Conclusion: Two-hour urine proteins can be used for assessment of proteinuria in preeclampsia instead of gold standard 24-hour urine collection for early diagnosis and better patient compliance. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/200 https://jfrh.tums.ac.ir/index.php/jfrh/article/download/200/195