Tehran University of Medical Sciences Journal of Family and Reproductive Health 1735-8949 14 2 2020 10 18 68 73 Zinat Ghanbari Department of Obstetrics & Gynecology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Samira Sohbati Department of Obstetrics & Gynecology, Kerman University of Medical Sciences, Kerman, Iran Tahereh Eftekhar Department of Obstetrics & Gynecology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Leyla Sahebi Maternal Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran Soodabeh Darvish Department of Obstetrics & Gynecology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Safa Alasiri Department of Obstetrics & Gynecology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Maryam Deldar Pasikhani Department of Obstetrics & Gynecology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran 2019 12 25 2020 07 20 Objective: The aim of this study was to evaluate the safety and efficacy of fractional CO2 laser for treatment of vulvovaginal atrophy. Materials and methods: In this clinical trial study, we evaluated the laser effect on vulvovaginal atrophy in 47 women aged 43-80 years. Each woman was treated with fractional CO2 laser, SmartXide2V2LR, DEKA, Monalisa Touch, vaginal 360o probe and cosmetic probe. The severity of vulvovaginal atrophy symptoms was evaluated before first session of laser and every 4 weeks after laser treatment until 12 weeks. Adverse effects including pain and itching were assessed at all visits. The statistical analysis was performed using SPSS version 23. Results: The response to laser therapy for vaginal dryness was significantly (p <0.001) improved. Also, the response to laser therapy for each symptom include dyspareunia, vaginal discharge, itching and urge urinary incontinence was statistically significant (p <0.001) based on visual analogue scale (VAS). Conclusion: It seems vaginal fractional CO2 laser can be applied as an effective and safe treatment method in genitourinary syndrome of menopause (GSM). It is necessary to conduct studies with long-term follow-up. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/1277 https://jfrh.tums.ac.ir/index.php/jfrh/article/download/1277/517