Tehran University of Medical Sciences Journal of Family and Reproductive Health 1735-8949 14 1 2020 07 18 38 44 Yulia Silvani Midwifery Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia Agnestia Dian Lovita Midwifery Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia Afniari Maharani Midwifery Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia I Wayan Wiyasa Department of Obstetrics and Gynecology, Dr. Saiful Anwar General Hospital, Malang, Indonesia Hidayat Sujuti Department of Biochemistry-Molecular Biology, Faculty of Medicine, University of Brawijaya, Malang, Indonesia Retty Ratnawati Department of Physiology, Faculty of Medicine University of Brawijaya, Malang, Indonesia Tri Yudani Raras Department of Biochemistry-Molecular Biology, Faculty of Medicine, University of Brawijaya, Malang, Indonesia 2019 09 24 2020 03 14 Objective: This study aimed to determine the effect of Extra Virgin Olive Oil (EVOO) on vasodilator enzyme by repairing angiogenic function in rat model of preeclampsia. Materials and methods: This research consisted of five groups; negative control (normal pregnant rats) group, positive control (preeclampsia rat model) group, preeclampsia rat model groups given EVOO in 3 different doses (0.5 ml/day, 1 ml/day, and 2 ml/day, respectively). Blood pressure measurements were carried out on day 12, 15, and 19 of pregnancy. After the rats were sacrificed, the placentas were collected to determine endothelial Nitric Oxide Synthase (eNOS) level of maternal plasma to determine soluble Fms-like Tyrosine Kinase 1 (sFlt-1) and Vascular Endothelial Growth Factor (VEGF) level. Results: There were significant higher sFlt-1 level (p < 0.001), lower VEGF level (p = 0.009), and lower eNOS level (p = 0.034) between negative and positive control groups. After EVOO administration, sFlt-1 level was lower in dose 1 and 2 groups but higher in dose 3 group in accordance with VEGF and eNOS levels that were increasing both in dose 1 and dose 2 groups but decreasing in dose 3. There were significant differences between positive control and dose 1 (p = 0.015) and dose 2 (p = 0.001) in sFlt-1 level. None of all dose groups were statistically different with positive control group in VEGF level (dose 1 p = 0.601; dose 2 p = 0.297; dose 3 p = 0.805). eNOS levels of all dose groups were statistically different from that of the positive control group (dose 1 p = 0.014; dose 2 p = 0.001; dose 3 p = 0.024). Conclusion: Administration of EVOO modulates eNOS as vasodilator enzyme by repairing the angiogenic function indicated by decreased sFlt-1 level and increased VEGF in rat model of preeclampsia. https://jfrh.tums.ac.ir/index.php/jfrh/article/view/1227 https://jfrh.tums.ac.ir/index.php/jfrh/article/download/1227/511