Journal of Family and Reproductive Health 2007. 1(1):47-50.

Antisperm antibody formation following Vasectomy
Mohammad Reza Nowroozi, Keivan Radkhah, Abdolhossein Keyhani, Mohsen Ayati, Hassan Jamshidian


Objective: Seeking for fertility after previous vasectomy is increasing. Vasectomy may break the blood testis barrier and in this way the level of antisperm antibody increases. It is suggested that increase in antisperm antibody decreases spontaneous pregnancy even after successful vasovasostomy. In this study we evaluated antisperm antibody formation 3 months after vasectomy.
Method: This study was performed on 80 healthy men requesting vasectomy from eurology ward of Imam Khomeini hospital. Blood antisperm antibody was checked at the time of vasectomy and 3 months later, when they came to assure vasectomy results, using Kibrick and sensitized RBCs.
Results: Only 2 patients had antisperm antibody before surgery. Three months after vasectomy 56.49% of the patients had antisperm antibody in Kibrick Method, and 53.56% of them had antisperm antibody in sensitized RBCs.
Conclusion: Vasectomy increases antisperm antibody level. We suggest evaluation of antisperm antibody before vasovasostomy to estimate the risk of infertility after successful procedure.


vasectomy; antisperm antibody; vasovasostomy

Full Text:



-Goldacre M, Vessey M, Clarke J, Heasman M. Record linkage study of morbidity following vasectomy. In: Lepow IH, Crozier R ,eds. Vasectomy: Immunologic and Pathophysiologic effects in Animals and Man. New York: Academic Press, 1979: 567-575.

-Massey FJ Jr, Bernstein GS, O’Fallon WM, Schuman LM,Coulson AH, Crozier R, et al. Vasectomy and health Results from a large cohort study. J Am Med Assoc 1984; 252: 1023-1029.

-Thomas AJ Jr, Pontes JE, Rose NR, Segal s, Pierce JM Jr. Microsurgical vasovasostomy: immunologic consequences and subsequent fertility. Fertil Steril 1981; 35:447-50.

-Martin DC. Microsurgical reversal of vasectomy. Am J Surg 1981; 142 :48-50.

-Mumford D, Ansbacher R, Sung JS. Longitudinal immune studies in vasectomized men. In: Lepow IH, Crozier R, eds. Vasectomy: Immunologic and Pathophysiologic Effects in Animals and Man. New York: Academic Press, 1979: 491-508.

-Linnet L , Suominen JJO. A comparison of eight techniques for the evaluation of the autoimmune response to spermatozoa after vasectomy. J Reprod Immunol 1982; 4: 133-144.

-Law HY, Bodmer WF, Mathews JD, Skegg DCG. The immune response to vasectomy and its relation to the HLA system. Tissue Antigens 1979; 14: 115-139.

-Rose NR, Lucas PL. Immunological consequences of vasectomy II. Two-year summary of a prospective study. In: Lepow IH, Crozier R, eds. Vasectomy: Immunologic and Pathophysiologic Effects in Animals and Man. New York: Academic Press, 1979: 533-560.

-Tung KS. Human sperm antigens and antisperm antibodies. I. Studies on vasectomized patients. Clin Exp Immunol 1975; 20: 93-104.

-Gerrad ER, Sandlow JI, Oster RA, Burns JR, Box LC, Kolletis PN. Effect of female partner age on pregnancy rates after vasectomy reversal. Fertil Steril 2007; 87(6): 1340-1344.

-Dole GR, Smith M. Microsurgical vasovasostomy at the Erasmus MC, 1998-2002: results and predictive factors. Ned Tijdschr Geneeskd 2005; 149(49): 2743-2747.

-Kay DJ, Clifton V, Taylor JS, Boettcher B. Anti-sperm antibodies and semen profiles in re-anastomosed men. Reprod Fertil Dev 1993; 5(1): 135-139.

-Francavilla F, Santucci R, Barbonetti A, Francavilla S. Naturally-occurring antisperm antibodies in men: interference with fertility and clinical implications. An update. Front Biosci 2007; 12: 2890-2911.

-Flickinger CJ, Howards SS, Bush LA, Baker LA, Herr JC. Temporal recognition of sperm autoantigens by IgM and IgG autoantibodies after vasectomy and vasovasostomy. J Reprod Immunol 1994; 27(2): 135-150.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.